Document 2061
 DOCN  M94A2061
 TI    The role of adhesion molecules in HIV infection.
 DT    9412
 AU    Gomez PM; Hildreth EK; Johns Hopkins University, School of Medicine,
       Department of; Pharmacology and Mol Sciences, Baltimore, Maryland 21205.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):39 (abstract no. 129A). Unique
       Identifier : AIDSLINE ICA10/94370514
 AB    OBJECTIVE: The goal of this research is to determine the role that
       adhesion molecules play in HIV-1 infection of primary cells. METHODS:
       HIV-1 isolates RF and MN were grown in Jurkat cells and used to infect
       PHA stimulated peripheral blood mononuclear cells. Plasma samples
       obtained from two normal vaccinated individuals hyperimmunized with
       recombinant HIV/MN gp120 from Genetech were tested in neutralization
       assays to determine their ability to neutralize the HIV/MN lab strain.
       Antibodies to CD4 and LFA-1 were used in combination with vaccinated
       sera to determine synergistic effect of inhibition. HIV production was
       determined by assaying for the viral core antigen (p24) by ELISA. Data
       is represented as percent of inhibition in comparison to virus
       production when no antibodies are present. RESULTS: Vaccinated sera V17
       and V424 were diluted 1:4 and 1:40 and cultured with HIV/MN and PHA
       blasts in the presence and absence of LFA-1 antibody (H52 20 ug/ml). As
       displayed in the graph there was a synergistic inhibition of viral
       replication when HIV was cultured in the presence of vaccinated sera and
       LFA-1 antibody. In addition, the effect of antibody to LFA-1 was the
       same regardless of the dilution of vaccinated sera. CONCLUSION: We
       conclude that adhesion molecules can play a role in HIV infection. HIV
       has been reported to acquire molecules which are expressed on the host
       cell surface during budding. Therefore, the quantity and the type of
       molecules acquired by the virus would be determined by the type of cell
       it buds. We speculate that primary isolates would acquire a greater
       degree of adhesion molecules because they would be budding from
       activated cells. We are currently performing neutralization assays to
       determine if vaccinated sera and adhesion molecules would neutralize
       primary isolates to a greater extent than lab-isolates. TABULAR DATA,
       SEE ABSTRACT VOLUME.
 DE    AIDS Vaccines/IMMUNOLOGY  Cell Adhesion Molecules/*IMMUNOLOGY/ISOLATION
       & PURIF  Enzyme-Linked Immunosorbent Assay  Human  HIV Core Protein
       p24/IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Leukocytes, Mononuclear/MICROBIOLOGY
       Lymphocyte Function-Associated Antigen-1/IMMUNOLOGY  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).